cell division and mitosis flip book list of events

Furthermore, we found that the main trigger is a mitotic defect, in particular, chromosome missegregation during bipolar mitosis or spindle multipolarity.

Here we show by live cell imaging of tetraploid cells generated by an induced cytokinesis failure that most tetraploids arrest and die in a p53-dependent manner after the first tetraploid mitosis. The trigger of this arrest has not been identified so far. An increasing body of evidence shows that the propagation of mammalian tetraploid cells is limited by a p53-dependent arrest. Kuffer, Christian Kuznetsova, Anastasia Yurievna StorchovÃ¡, ZuzanaĮrroneously arising tetraploid mammalian cells are chromosomally instable and may facilitate cell transformation. All rights reserved.Ībnormal mitosis triggers p53-dependent cell cycle arrest in human tetraploid cells. We show that positive feedback is important to keep mitosis short, constant, and temporally insulated and anticipate it might be a commonly used regulatory strategy to create modularity in other biological systems. Perturbing positive feedback gave rise to a sluggish, variable entry and progression through mitosis and uncoupled duration of mitosis from variability in cell cycle length. By combining live cell imaging and computational modeling, we showed that positive feedback is the molecular mechanism underlying the temporal insulation of mitosis.

Surprisingly, there is no correlation between cell-cycle length and mitotic duration, suggesting that mitosis is temporally insulated from variability in earlier cell-cycle phases. Quantitative measurements of cell-cycle dynamics in single cells showed that despite variability in G1-, S-, and G2 phases, duration of mitosis is short and remarkably constant. Positive Feedback Keeps Duration of Mitosis Temporally Insulated from Upstream Cell-Cycle Events.Īraujo, Ana Rita Gelens, Lendert Sheriff, Rahuman S M Santos, Silvia D MĬell division is characterized by a sequence of events by which a cell gives rise to two daughter cells.

Cell division and mitosis flip book list of events